A cytotoxic effect of tomato extracts was observed only after processing. This result well correlates with the flavonoid content after processing and clearly indicates that processed yellow tomatoes have a high content of bioactive compounds endowed with cytotoxicity towards cancer cells, thus opening the way to obtain tomato-based functional foods.  According to this hypothesis, it has been reported that in HepG2 cells chlorogenic acid has no cytotoxic effect and improves cellular tolerance against oxidative factors, by activating survival/proliferation pathways. Finally, the higher cell growth inhibition observed with E87 and E92 tomato extracts after processing, in particular on Hek293 cells, could be due to the presence of higher naringenin levels in these samples. This flavanone is known to induce cytotoxicity and apoptosis in various human cancer cells. These results have been confirmed by trypan blue dye exclusion assay. Overall, even after debittering, the lupin seeds preserved a significant content of phenolic compounds, which are positively correlated to the antioxidant capacity. The citric acid solutions better preserved the conjugated phenols (especially using the shortest soaking time), while the NaCl solutions behaved poorly. The contrary was instead evident for the bound phenols; all of the samples that were treated with salt solutions (either by the control method or by the experimental protocol) showed significantly higher levels of insoluble-bound polyphenols compared to the samples that were treated with the traditional method or with citric acid The treatment with 1% NaCl for 45 h retained the greatest amount of bounds phenols. Future investigations may take advantage of our results by assessing the bioaccessibility of the bioactive nutrients in commercial-like products from lupin flour. Furthermore, the experimental debittering method may be applied to germinated lupin seeds because the germination triggers the synthesis of antioxidants in legumes; however, conclusive evidence is still missing in lupins. it is important to provide micronutrients to crops in order to reduce micronutrient malnutrition globally.  According to some research, iron insufficiency is the most prevalent micronutrient imbalance in human populations. Fe is important for a variety of processes, including DNA synthesis, respiration, photosynthesis, the electron transport chain, and nitrogen reduction but is a scarce nutrient for plants in alkaline soil.